<i>Helicobacter acinonychis</i> : Genetic and Rodent Infection Studies of a <i>Helicobacter pylori</i> -Like Gastric Pathogen of Cheetahs and Other Big Cats

DOI Web Site 被引用文献1件
  • Daiva Dailidiene
    Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110
  • Giedrius Dailide
    Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110
  • Keiji Ogura
    Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110
  • Maojun Zhang
    Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110
  • Asish K. Mukhopadhyay
    Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110
  • Kathryn A. Eaton
    Department of Veterinary Biosciences, Ohio State University, Columbus, Ohio
  • Giovanni Cattoli
    Department of Medical Microbiology, School of Medicine, Vrije Universitet Medical Center, Amsterdam
  • Johannes G. Kusters
    Department of Gastroenterology and Hepatology, Erasmus MC—University Medical Center Rotterdam, Rotterdam, The Netherlands
  • Douglas E. Berg
    Departments of Molecular Microbiology and Genetics, Washington University Medical School, St. Louis, Missouri 63110

この論文をさがす

説明

<jats:title>ABSTRACT</jats:title> <jats:p> Insights into bacterium-host interactions and genome evolution can emerge from comparisons among related species. Here we studied <jats:italic>Helicobacter acinonychis</jats:italic> (formerly <jats:italic>H. acinonyx</jats:italic> ), a species closely related to the human gastric pathogen <jats:italic>Helicobacter pylori</jats:italic> . Two groups of strains were identified by randomly amplified polymorphic DNA fingerprinting and gene sequencing: one group from six cheetahs in a U.S. zoo and two lions in a European circus, and the other group from a tiger and a lion-tiger hybrid in the same circus. PCR and DNA sequencing showed that each strain lacked the <jats:italic>cag</jats:italic> pathogenicity island and contained a degenerate vacuolating cytotoxin ( <jats:italic>vacA</jats:italic> ) gene. Analyses of nine other genes ( <jats:italic>glmM</jats:italic> , <jats:italic>recA</jats:italic> , <jats:italic>hp519</jats:italic> , <jats:italic>glr</jats:italic> , <jats:italic>cysS</jats:italic> , <jats:italic>ppa</jats:italic> , <jats:italic>flaB</jats:italic> , <jats:italic>flaA</jats:italic> , and <jats:italic>atpA</jats:italic> ) revealed a ∼2% base substitution difference, on average, between the two <jats:italic>H. acinonychis</jats:italic> groups and a ∼8% difference between these genes and their homologs in <jats:italic>H. pylori</jats:italic> reference strains such as 26695. <jats:italic>H. acinonychis</jats:italic> derivatives that could chronically infect mice were selected and were found to be capable of persistent mixed infection with certain <jats:italic>H. pylori</jats:italic> strains. Several variants, due variously to recombination or new mutation, were found after 2 months of mixed infection. <jats:italic>H. acinonychis</jats:italic> ' modest genetic distance from <jats:italic>H. pylori</jats:italic> , its ability to infect mice, and its ability to coexist and recombine with certain <jats:italic>H. pylori</jats:italic> strains in vivo should be useful in studies of <jats:italic>Helicobacter</jats:italic> infection and virulence mechanisms and studies of genome evolution. </jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ