Detailed Description of a Cranial Window Technique for Acute and Chronic Experiments

  • JOSEPH E. LEVASSEUR
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Health Sciences Division, 1200 East Broad Street, Richmond, Virginia 23298
  • ENOCH P. WEI
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Health Sciences Division, 1200 East Broad Street, Richmond, Virginia 23298
  • A. JARRELL RAPER
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Health Sciences Division, 1200 East Broad Street, Richmond, Virginia 23298
  • HERMES A. KONTOS
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Health Sciences Division, 1200 East Broad Street, Richmond, Virginia 23298
  • JOHN L. PATTERSON
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Health Sciences Division, 1200 East Broad Street, Richmond, Virginia 23298

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説明

<jats:p>Methods for implantation of cranial windows for the direct observation of the pial microcirculation in experimental animals are described in detail. These techniques are suitable for both acute experiments in anesthetized animals and chronic implantation permitting several months of observation in awake animals. Experience over several years shows that these techniques have an acceptably low rate of failure, are low in cost and can easily be mastered in most laboratories. They make possible observation of the microcirculation and accurate measurement of the diameter of pial vessels, and permit study of the effects on the microcirculation of a variety of maneuvers and vasoactive agents which can be studied by direct application as well as by intravascular administration. Because they preserve the integrity of the skull, the techniques permit study of the cerebral microcirculation under conditions closely approximating the normal environment of these vessels.</jats:p>

収録刊行物

  • Stroke

    Stroke 6 (3), 308-317, 1975-05

    Ovid Technologies (Wolters Kluwer Health)

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