Water‐Soluble, Thermostable, Photomodulated Color‐Switching Quantum Dots

DOI Web Site Web Site 被引用文献4件
  • Sebastián A. Díaz
    Center for Bio/Molecular Science and Engineering, Code 6900 U.S. Naval Research Laboratory Washington, DC 20375 USA
  • Florencia Gillanders
    Center for Investigation in Bionanosciences (CIBION-CONICET) Buenos Aires Argentina
  • Kimihiro Susumu
    Optical Sciences Division, Code 5611 U.S. Naval Research Laboratory Washington, DC 20375 USA
  • Eunkeu Oh
    Optical Sciences Division, Code 5611 U.S. Naval Research Laboratory Washington, DC 20375 USA
  • Igor L. Medintz
    Center for Bio/Molecular Science and Engineering, Code 6900 U.S. Naval Research Laboratory Washington, DC 20375 USA
  • Thomas M. Jovin
    Laboratory of Cellular Dynamics Max Planck Institute for Biophysical Chemistry 37077 Göttingen Germany

書誌事項

公開日
2016-11-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/chem.201604688
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>Photoswitchable probes are of great utility in fluorescence microscopy, permitting numerous determinations, including molecular localization for super‐resolution, based on their modifiable emission intensity and spectra. We have coated a blue‐emitting (425 nm) quantum dot (QD) with a diheteroarylethene photochrome (PCf), the closed form isomer of which has absorption and emission maxima at 440 and 520–530 nm, respectively, and thus functions as a fluorescent acceptor for the QD donor in Förster resonance energy transfer (FRET). The transition from the non‐absorbing, non‐fluorescent open state to the fluorescent closed state is achieved by irradiation in the near‐UV and reversed by visible light. The PCf is coupled to an amphiphilic polymer that stably coats the QD, thereby creating a water‐soluble color‐switching QD (csQD) emitting in the blue after visible light irradiation and in the green after UV irradiation. Thus, csQDs photomodulate between two observable states, without the “off” state of previous constructs. The resulting change in the emission ratios of the QD and PCf is up to 180 %, and the csQD can undergo multiple photocycles with minimal fatigue.</jats:p>

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