Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip
-
- Hyun Jung Kim
- Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;
-
- Hu Li
- Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;
-
- James J. Collins
- Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;
-
- Donald E. Ingber
- Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;
書誌事項
- 公開日
- 2015-12-14
- DOI
-
- 10.1073/pnas.1522193112
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:title>Significance</jats:title> <jats:p>The main advance of this study is the development of a microengineered model of human intestinal inflammation and bacterial overgrowth that permits analysis of individual contributors to the pathophysiology of intestinal diseases, such as ileus and inflammatory bowel disease, over a period of weeks in vitro. By studying living human intestinal epithelium, with or without vascular and lymphatic endothelium, immune cells, and mechanical deformation, as well as living microbiome and pathogenic microbes, we identified previously unknown contributions of specific cytokines, mechanical motions, and microbiome to intestinal inflammation, bacterial overgrowth, and control of barrier function. We provide proof-of-principle to show that the microfluidic gut-on-a-chip device can be used to create human intestinal disease models and gain new insights into gut pathophysiology.</jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 113 (1), E7-, 2015-12-14
Proceedings of the National Academy of Sciences