A facile technology for the high-throughput sequencing of the paired VH:VL and TCRβ:TCRα repertoires
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- Hidetaka Tanno
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Jonathan R. McDaniel
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Christopher A. Stevens
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- William N. Voss
- Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
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- Jie Li
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Russell Durrett
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Jiwon Lee
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Jimmy Gollihar
- Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA.
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- Yuri Tanno
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- George Delidakis
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Arti Pothukuchy
- Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
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- Jared W. Ellefson
- Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
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- Jörg J. Goronzy
- Division of Immunology and Rheumatology, Department of Medicine, Stanford University, Stanford, CA, USA.
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- Jennifer A. Maynard
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
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- Andrew D. Ellington
- Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
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- Gregory C. Ippolito
- Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
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- George Georgiou
- Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
抄録
<jats:p>We report a simple and low-cost one-step high-throughput technology for sequencing the paired antibody/TCR repertoire.</jats:p>
収録刊行物
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- Science Advances
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Science Advances 6 (17), 2020-04-24
American Association for the Advancement of Science (AAAS)