The Mtr Pathway of <i>Shewanella oneidensis</i> MR‐1 Couples Substrate Utilization to Current Production in <i>Escherichia coli</i>
抄録
<jats:title>Abstract</jats:title><jats:p>Introducing an electronic interface into <jats:italic>Escherichia coli</jats:italic> will allow its enormous synthetic biology toolkit to be leveraged in bioelectrochemical applications. While <jats:italic>E. coli</jats:italic> expressing the Mtr pathway of <jats:italic>Shewanella oneidensis</jats:italic> MR‐1 transfer electrons to an anode, it has remained unclear if this current production alters the intracellular state of <jats:italic>E. coli</jats:italic>, which is a critical requirement for bioelectronic technologies. Here we address this by characterizing current production in Mtr‐expressing <jats:italic>E. coli</jats:italic> and its effects on cellular viability, substrate consumption, and product generation. We found that <jats:italic>cymA‐mtr E. coli</jats:italic> sustained ∼8‐fold higher current levels than a control strain. This increased current production did not change <jats:italic>E. coli</jats:italic> viability or substrate consumption, but it did alter metabolic fluxes. A shift to more oxidized products strongly suggests that the Mtr pathway improves redox balance in <jats:italic>E. coli</jats:italic>. By demonstrating the Mtr module couples current production to intracellular state, this work establishes Mtr‐expressing <jats:italic>E. coli</jats:italic> as a platform for accelerated development of bioelectronic technologies.</jats:p>
収録刊行物
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- ChemElectroChem
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ChemElectroChem 1 (11), 1874-1879, 2014-08-29
Wiley