Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry
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- Amy Galvin
- HSCI‐CRM Flow Cytometry Core Facility, Center for Regenerative Medicine, Massachusetts General Hospital Boston Massachusetts
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- Meredith Weglarz
- Stanford Shared FACS Facility Stanford California
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- Kat Folz‐Donahue
- FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing Cologne Germany
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- Maris Handley
- HSCI‐CRM Flow Cytometry Core Facility, Center for Regenerative Medicine, Massachusetts General Hospital Boston Massachusetts
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- Misa Baum
- Fred Hutchinson Cancer Research Center Seattle Washington
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- Michael Mazzola
- Center for Regenerative Medicine, Massachusetts General Hospital Boston Massachusetts
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- Hannah Litwa
- NYU Langone Medical Center New York New York
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- David T. Scadden
- Center for Regenerative Medicine, Massachusetts General Hospital Boston Massachusetts
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- Lev Silberstein
- Fred Hutchinson Cancer Research Center Seattle Washington
抄録
<jats:title>Abstract</jats:title><jats:p>Maintenance of hematopoietic stem cell (HSC) quiescence is critical for self‐renewal and differentiation into mature lineages. Therefore, the ability to reliably detect abnormal HSC cycling is essential for experiments that seek to investigate abnormalities of HSC function. The ability to reproducibly evaluate cell cycle status in a rare cell subset requires careful optimization of multiple parameters during cell preparation and sample processing. Here, we describe a method where data acquisition parameters and fluorochrome combination for long‐term HSC staining have been specifically designed for concurrent use with DAPI and Ki‐67 antibodies. © 2018 by John Wiley & Sons, Inc.</jats:p>
収録刊行物
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- Current Protocols in Cytometry
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Current Protocols in Cytometry 87 (1), e50-, 2018-10-18
Wiley
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詳細情報 詳細情報について
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- CRID
- 1360013172695599872
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- DOI
- 10.1002/cpcy.50
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- ISSN
- 19349300
- 19349297
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- データソース種別
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- Crossref