Species-specific antigens in salivary glands of phlebotomine sandflies

書誌事項

公開日
2001-01
権利情報
  • https://www.cambridge.org/core/terms
DOI
  • 10.1017/s0031182000007046
公開者
Cambridge University Press (CUP)

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説明

<jats:p>Saliva inoculated by sandfly females during feeding stimulated production of high levels of anti-saliva antibodies. To determine whether 3 species of the genus <jats:italic>Phlebotomus</jats:italic> have species-specific salivary antigens we performed dot-blots and immunoblots using sera from mice, hamsters and rabbits repeatedly bitten by sandflies. Important differences were found in the antigen components of the salivary gland lysates (SGL) of <jats:italic>Phlebotomus papatasi, P. perniciosus </jats:italic> and <jats:italic>P. halepensis.</jats:italic> In total 4–9 species-specific antigens were detected in each species by immunoblotting. Cross-reactivity was not detected between <jats:italic>P. papatasi</jats:italic> and the other species tested; in the SGL of <jats:italic>P. papatasi</jats:italic> sera from animals bitten by this species recognized 5–7 major antigens while sera from animals bitten by other species did not react. A weak cross-reaction was observed between <jats:italic>P. perniciosus</jats:italic> and <jats:italic>P. halepensis</jats:italic>; in SGL from <jats:italic>P. perniciosus</jats:italic>, the sera from rabbits and hamsters bitten by this species recognized about 8 intense bands while sera from animals bitten by <jats:italic>P. halepensis</jats:italic> reacted weakly with up to 4 saliva polypeptides.</jats:p>

収録刊行物

  • Parasitology

    Parasitology 122 (1), 37-41, 2001-01

    Cambridge University Press (CUP)

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