Cascaded Biocatalysis and Bioelectrocatalysis: Overview and Recent Advances

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  • Yoo Seok Lee
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA;
  • Koun Lim
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA;
  • Shelley D. Minteer
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA;

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<jats:p> Enzyme cascades are plentiful in nature, but they also have potential in artificial applications due to the possibility of using the target substrate in biofuel cells, electrosynthesis, and biosensors. Cascade reactions from enzymes or hybrid bioorganic catalyst systems exhibit extended substrate range, reaction depth, and increased overall performance. This review addresses the strategies of cascade biocatalysis and bioelectrocatalysis for ( a) CO<jats:sub>2</jats:sub> fixation, ( b) high value-added product formation, ( c) sustainable energy sources via deep oxidation, and ( d) cascaded electrochemical enzymatic biosensors. These recent updates in the field provide fundamental concepts, designs of artificial electrocatalytic oxidation-reduction pathways (using a flexible setup involving organic catalysts and engineered enzymes), and advances in hybrid cascaded sensors for sensitive analyte detection. </jats:p>

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