Characterization of tulip streak virus, a novel virus associated with the family Phenuiviridae

DOI Web Site 2 Citations
  • Yutaro Neriya
    School of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan
  • Toshiyuki Morikawa
    Toyama Prefectural Agricultural, Forestry and Fisheries Research Center, Yoshioka 1124-1, Toyama, Toyama 939-8153, Japan
  • Kakeru Hamamoto
    School of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan
  • Kengo Noguchi
    School of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan
  • Tominari Kobayashi
    School of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan
  • Tomohiro Suzuki
    Center for Bioscience Research and Education, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan
  • Hisashi Nishigawa
    School of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan
  • Tomohide Natsuaki
    School of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya, Tochigi 321-8505, Japan

Abstract

<jats:p>In Japan, tulip-growing areas have been plagued by viral diseases for decades, but the viruses causing the damage remain undescribed. In this study, <jats:italic>Nicotiana benthamiana</jats:italic> and <jats:italic>Chenopodium quinoa</jats:italic> plants mechanically inoculated with crude sap from a symptomatic tulip flower exhibited necrosis symptoms. Additionally, flexuous and filamentous virus particles were detected by electron microscopy analysis. Moreover, we determined the complete sequences of two genomic segments of the tulip streak virus (TuSV), which is a new virus associated with streaking symptoms, on the basis of a next-generation sequencing analysis. Homology analyses of the amino acid sequence of RNA-dependent RNA polymerase and the terminal sequence of the genomic RNA indicated that TuSV is associated with viruses in the family <jats:italic>Phenuiviridae</jats:italic>, but differs substantially from other reported viruses.</jats:p>

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