Imaging the invisible—Bioorthogonal Raman probes for imaging of cells and tissues

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  • Georgette Azemtsop Matanfack
    Institute of Physical Chemistry and Abbe Center of Photonics (IPC), Friedrich‐Schiller‐University Jena Jena Germany
  • Jan Rüger
    Leibniz Institute of Photonic Technology ‐ a member of the Leibniz Research Alliance Leibniz Health Technology (Leibniz‐IPHT) Jena Germany
  • Clara Stiebing
    Leibniz Institute of Photonic Technology ‐ a member of the Leibniz Research Alliance Leibniz Health Technology (Leibniz‐IPHT) Jena Germany
  • Michael Schmitt
    Institute of Physical Chemistry and Abbe Center of Photonics (IPC), Friedrich‐Schiller‐University Jena Jena Germany
  • Jürgen Popp
    Institute of Physical Chemistry and Abbe Center of Photonics (IPC), Friedrich‐Schiller‐University Jena Jena Germany

書誌事項

公開日
2020-07-23
権利情報
  • http://creativecommons.org/licenses/by-nc-nd/4.0/
DOI
  • 10.1002/jbio.202000129
公開者
Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>A revolutionary avenue for vibrational imaging with super‐multiplexing capability can be seen in the recent development of Raman‐active bioortogonal tags or labels. These tags and isotopic labels represent groups of chemically inert and small modifications, which can be introduced to any biomolecule of interest and then supplied to single cells or entire organisms. Recent developments in the field of spontaneous Raman spectroscopy and stimulated Raman spectroscopy in combination with targeted imaging of biomolecules within living systems are the main focus of this review. After having introduced common strategies for bioorthogonal labeling, we present applications thereof for profiling of resistance patterns in bacterial cells, investigations of pharmaceutical drug‐cell interactions in eukaryotic cells and cancer diagnosis in whole tissue samples. Ultimately, this approach proves to be a flexible and robust tool for in vivo imaging on several length scales and provides comparable information as fluorescence‐based imaging without the need of bulky fluorescent tags.<jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/jbio202000129-gra-0001.png" xlink:title="image"/></jats:p>

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