A metagenomic study of biliary microbiome change along the cholecystitis‐carcinoma sequence
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- Xiaoling Song
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Xu'an Wang
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Yunping Hu
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Huaifeng Li
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Tai Ren
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Yongsheng Li
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Liguo Liu
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Lin Li
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Xuechuan Li
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Ziyi Wang
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Wen Huang
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Runfa Bao
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Yijian Zhang
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Maolan Li
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Xuefeng Wang
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Feng Liu
- Emergency Unit, The First Affiliated Hospital Nanchang University Nanchang China
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- Jun Gu
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Linhui Zheng
- Emergency Unit, The First Affiliated Hospital Nanchang University Nanchang China
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- Wenguang Wu
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
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- Yingbin Liu
- Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
説明
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Gallbladder cancer (GBC) is the most common cancer type of the biliary tract, and an association has been found between chronic calculous cholecystitis (CCC) and an increased incidence of GBC mortality. An understanding of the relationship between CCC and its carcinogenesis may enable us to prevent and cure GBC. In this study, we attempted to explore changes in the microbiome profile that take place during the transition from chronic cholecystitis mucosa to malignant lesions.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Seven paired human GBC and CCC samples were provided by patients who had undergone laparoscopic cholecystectomy or radical cholecystectomy. Mucosal DNA extraction and metagenomic sequencing were performed to evaluate changes in the microbiota between the two groups. We found that GBC patients and CCC patients shared similar stable and permanent dominant species and showed apparent differences in their biliary microbial composition and gene function. <jats:italic>Peptostreptococcus stomatis</jats:italic> and <jats:italic>Enterococcus faecium</jats:italic> may potentially play a role in GBC progression. In addition, the metagenomic species profiles, co‐abundance and co‐exclusion correlations, and CAZyme prevalence showed significant differences between the CCC and GBC groups.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Our data suggested that changes in the microbiota between CCC and GBC may help deepen our understanding of the complex spectrum of different microbiotas involved in the development of GBC. Although the cohort size was small, this study has presented the first evidence of the existence of an altered biliary microbiota in GBC, which is clearly different from that in CCC patients.</jats:p></jats:sec>
収録刊行物
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- Clinical and Translational Medicine
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Clinical and Translational Medicine 10 (2), 2020-06
Wiley
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詳細情報 詳細情報について
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- CRID
- 1360016870126492032
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- DOI
- 10.1002/ctm2.97
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- ISSN
- 20011326
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- データソース種別
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- Crossref