Cancer-specific tissue-resident memory T-cells express ZNF683 in colorectal cancer
抄録
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>Tissue-resident memory T (Trm) cells are associated with cytotoxicity not only in viral infection and autoimmune disease pathologies but also in many cancers. Tumour-infiltrating CD103<jats:sup>+</jats:sup> Trm cells predominantly comprise CD8 T cells that express cytotoxic activation and immune checkpoint molecules called exhausted markers. This study aimed to investigate the role of Trm in colorectal cancer (CRC) and characterise the cancer-specific Trm.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>Immunochemical staining with anti-CD8 and anti-CD103 antibodies for resected CRC tissues was used to identify the tumour-infiltrating Trm cells. The Kaplan–Meier estimator was used to evaluate the prognostic significance. Cells immune to CRC were targeted for single-cell RNA-seq analysis to characterise cancer-specific Trm cells in CRC.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>The number of CD103<jats:sup>+</jats:sup>/CD8<jats:sup>+</jats:sup> tumour-infiltrating lymphocytes (TILs) was a favourable prognostic and predictive factor of the overall survival and recurrence-free survival in patients with CRC. Single-cell RNA-seq analysis of 17,257 CRC-infiltrating immune cells revealed a more increased zinc finger protein 683 (ZNF683) expression in cancer Trm cells than in noncancer Trm cells and in high-infiltrating Trm cells than low-infiltrating Trm in cancer, with an upregulated T-cell receptor (TCR)- and interferon-γ (IFN-γ) signalling-related gene expression in ZNF683<jats:sup>+</jats:sup> Trm cells.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>The number of CD103<jats:sup>+</jats:sup>/CD8<jats:sup>+</jats:sup> TILs is a prognostic predictive factor in CRC. In addition, we identified the ZNF683 expression as one of the candidate markers of cancer-specific Trm cells. IFN-γ and TCR signalling and ZNF683 expression are involved in Trm cell activation in tumours and are promising targets for cancer immunity regulation.</jats:p> </jats:sec>
収録刊行物
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- British Journal of Cancer
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British Journal of Cancer 128 (10), 1828-1837, 2023-03-03
Springer Science and Business Media LLC
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詳細情報 詳細情報について
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- CRID
- 1360017280648751616
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- ISSN
- 15321827
- 00070920
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- データソース種別
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- Crossref
- KAKEN