Mutations in RZF1, a zinc-finger protein, reduce magnesium uptake in roots and translocation to shoots in rice

DOI PDF PDF 72 References Open Access
  • Natsuko I Kobayashi
    Graduate School of Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan
  • Hiroki Takagi
    Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University , 1-308 Suematsu, Nonoichi, Ishikawa 921-8836 , Japan
  • Xiaoyu Yang
    Graduate School of Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan
  • Ayako Nishizawa-Yokoi
    Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO) , 3-1-3 Kannondai, Tsukuba 305-8604 , Japan
  • Tenta Segawa
    Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University , 1-308 Suematsu, Nonoichi, Ishikawa 921-8836 , Japan
  • Tatsuaki Hoshina
    Graduate School of Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan
  • Takayuki Oonishi
    Center for Education and Research of Community Collaboration, Utsunomiya University , Utsunomiya 321-8505 , Japan
  • Hisashi Suzuki
    National Institutes for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555 , Japan
  • Ren Iwata
    Cyclotron and Radioisotope Center (CYRIC), Tohoku University , 6-3 Aramaki Aza-Aoba, Aoba-ku, Sendai 980-8572 , Japan
  • Seiichi Toki
    Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO) , 3-1-3 Kannondai, Tsukuba 305-8604 , Japan
  • Tomoko M Nakanishi
    Graduate School of Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan
  • Keitaro Tanoi
    Graduate School of Life Sciences, The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 , Japan

Abstract

<jats:title>Abstract</jats:title> <jats:p>Magnesium (Mg) homeostasis is critical for maintaining many biological processes, but little information is available to comprehend the molecular mechanisms regulating Mg concentration in rice (Oryza sativa). To make up for the lack of information, we aimed to identify mutants defective in Mg homeostasis through a forward genetic approach. As a result of the screening of 2,825 M2 seedlings mutated by ion-beam irradiation, we found a rice mutant that showed reduced Mg content in leaves and slightly increased Mg content in roots. Radiotracer 28Mg experiments showed that this mutant, named low-magnesium content 1 (LMGC1), has decreased Mg2+ influx in the root and Mg2+ translocation from root to shoot. Consequently, LMGC1 is sensitive to the low Mg condition and prone to develop chlorosis in the young mature leaf. The MutMap method identified a 7.4-kbp deletion in the LMGC1 genome leading to a loss of two genes. Genome editing using CRISPR-Cas9 further revealed that one of the two lost genes, a gene belonging to the RanBP2-type zinc-finger family that we named RanBP2-TYPE ZINC FINGER1 (OsRZF1), was the causal gene of the low Mg phenotype. OsRZF1 is a nuclear protein and may have a fundamental role in maintaining Mg homeostasis in rice plants.</jats:p>

Journal

  • Plant Physiology

    Plant Physiology 192 (1), 342-355, 2023-02-22

    Oxford University Press (OUP)

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