The eukaryotic way to defend and edit genomes by sRNA‐targeted DNA deletion
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- Estienne C. Swart
- Institute of Cell Biology University of Bern Bern Switzerland
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- Mariusz Nowacki
- Institute of Cell Biology University of Bern Bern Switzerland
書誌事項
- 公開日
- 2015-01-07
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/nyas.12636
- 公開者
- Wiley
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説明
<jats:p>While there is currently burgeoning interest in the application of the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR‐associated genes) to genome editing, it is perhaps not widely appreciated that this is the second discovery of a small RNA (sRNA)–targeted DNA‐deletion system. The first sRNA–targeted DNA‐deletion system to be discovered, which we call IES/Ias (internal eliminated sequence/IES‐associated genes) to contrast with CRISPR/Cas, is found in ciliates, and, like CRISPR/Cas, is thought to serve as a form of immune defense against invasive DNAs. The manner in which the ciliate IES/Ias system functions is distinct from that of the CRISPR/Cas system in archaea and bacteria, and arose independently through a synthesis of RNA interference–derived and DNA‐specific molecular components. Despite the major differences between CRISPR/Cas and IES/Ias, both systems face similar conceptual challenges in targeting invasive DNAs. In this review, we focus on the discovery, effects, function, and evolutionary consequences of the IES/Ias system.</jats:p>
収録刊行物
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- Annals of the New York Academy of Sciences
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Annals of the New York Academy of Sciences 1341 (1), 106-114, 2015-01-07
Wiley