<jats:p>Aim. We investigated different bioactive compounds including epigallocatechin gallate (EGCG), anthocyanidin, resveratrol, phloretin, spermidine, butyrate, and β-hydroxybutyrate with regard to their effect on SIRT3 via NRF2 and modulation of the proinflammatory senescence-associated secretory phenotype (SASP) in senescence induced 3T3-L1 preadipocytes. Methods. For induction of senescence, 3T3-L1 preadipocytes were incubated with bromodeoxyuridine (BrdU) for 8 days. Cell cycle inhibition was observed, and β-galactosidase activity was measured. After BrdU treatment, cells were treated with different bioactive compounds in various concentrations for 96 h. ELISA was used for determining proinflammatory cytokine IL6 in SASP cells. Results. CDKN1a increased significantly after BrdU incubation compared to untreated control (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M1"> <mi>p</mi> <mo><</mo> <mn>0.01</mn> </math> </jats:inline-formula>). All secondary plant ingredients used for treatment, but not anthocyanidin 50 μM, decrease CDKN1a expression (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M2"> <mi>p</mi> <mo><</mo> <mn>0.05</mn> </math> </jats:inline-formula>), whereas most endogenous substances did not attenuate CDKN1a. IL6 secretion positively correlated with CDKN1a (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M3"> <mi>p</mi> <mo><</mo> <mn>0.01</mn> </math> </jats:inline-formula>), whereas EGCG could diminish both, IL6 and CDKN1a with the strongest effect (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M4"> <mi>p</mi> <mo><</mo> <mn>0.01</mn> </math> </jats:inline-formula>). Although NRF2 positively correlated with SIRT3 activation (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M5"> <mi>p</mi> <mo><</mo> <mn>0.05</mn> </math> </jats:inline-formula>), only resveratrol (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M6"> <mi>p</mi> <mo><</mo> <mn>0.01</mn> </math> </jats:inline-formula>) and anthocyanidin (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M7"> <mi>p</mi> <mo><</mo> <mn>0.05</mn> </math> </jats:inline-formula>) could activate NRF2 significantly. Solely anthocyanidin 50 μM (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M8"> <mi>p</mi> <mo><</mo> <mn>0.05</mn> </math> </jats:inline-formula>) and 100 μM (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M9"> <mi>p</mi> <mo><</mo> <mn>0.01</mn> </math> </jats:inline-formula>) and EGCG 50 μM (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M10"> <mi>p</mi> <mo><</mo> <mn>0.01</mn> </math> </jats:inline-formula>) could increase SIRT3 expression. Activation of SIRT3 with EGCG correlated with lowered IL6 secretion significantly (<jats:inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M11"> <mi>p</mi> <mo><</mo> <mn>0.05</mn> </math> </jats:inline-formula>) but not with anthocyanidin. Conclusion. Accumulation of senescent cells in adipose tissue plays an important role in obesity and age-related diseases. SIRT3, located in the mitochondria, can regulate ROS via different pathways. Thus, targeting SIRT3 activating compounds such as EGCG may delay senescence of cells and senescence induced inflammatory processes.</jats:p>