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- Daniel Schraivogel
- Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
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- Terra M. Kuhn
- Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany.
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- Benedikt Rauscher
- Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
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- Marta Rodríguez-Martínez
- Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
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- Malte Paulsen
- Flow Cytometry Core Facility, EMBL, Heidelberg, Germany.
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- Keegan Owsley
- BD Biosciences, San Jose, CA, USA.
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- Aaron Middlebrook
- BD Biosciences, San Jose, CA, USA.
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- Christian Tischer
- Advanced Light Microscopy Core Facility, EMBL, Heidelberg, Germany.
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- Beáta Ramasz
- Flow Cytometry Core Facility, EMBL, Heidelberg, Germany.
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- Diana Ordoñez-Rueda
- Flow Cytometry Core Facility, EMBL, Heidelberg, Germany.
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- Martina Dees
- Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany.
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- Sara Cuylen-Haering
- Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany.
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- Eric Diebold
- BD Biosciences, San Jose, CA, USA.
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- Lars M. Steinmetz
- Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
抄録
<jats:p>Fast and selective isolation of single cells with unique spatial and morphological traits remains a technical challenge. Here, we address this by establishing high-speed image-enabled cell sorting (ICS), which records multicolor fluorescence images and sorts cells based on measurements from image data at speeds up to 15,000 events per second. We show that ICS quantifies cell morphology and localization of labeled proteins and increases the resolution of cell cycle analyses by separating mitotic stages. We combine ICS with CRISPR-pooled screens to identify regulators of the nuclear factor κB (NF-κB) pathway, enabling the completion of genome-wide image-based screens in about 9 hours of run time. By assessing complex cellular phenotypes, ICS substantially expands the phenotypic space accessible to cell-sorting applications and pooled genetic screening.</jats:p>
収録刊行物
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- Science
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Science 375 (6578), 315-320, 2022-01-21
American Association for the Advancement of Science (AAAS)