Dynamic Ligand Screening by Magnetic Nanoassembly Modulates Stem Cell Differentiation
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- Hyunsik Hong
- Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea
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- Sunhong Min
- Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea
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- Sagang Koo
- Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826 Republic of Korea
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- Yunjung Lee
- Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826 Republic of Korea
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- Jinho Yoon
- Department of Chemistry and Chemical Biology Rutgers University Piscataway NJ 08854 USA
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- Woo Young Jang
- Department of Orthopedic Surgery Korea University Anam Hospital Seoul 02841 Republic of Korea
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- Nayeon Kang
- Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea
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- Ramar Thangam
- Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea
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- Hyojun Choi
- Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea
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- Hee Joon Jung
- Department of Materials Science and Engineering Northwestern University Evanston IL 60208 USA
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- Seong‐Beom Han
- KU‐KIST Graduate School of Converging Science and Technology Korea University Seoul 02841 Republic of Korea
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- Qiang Wei
- College of Polymer Science and Engineering State Key Laboratory of Polymer Materials and Engineering Sichuan University Chengdu 610065 China
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- Seung‐Ho Yu
- Department of Chemical and Biological Engineering Korea University Seoul 02841 Republic of Korea
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- Dong‐Hwee Kim
- KU‐KIST Graduate School of Converging Science and Technology Korea University Seoul 02841 Republic of Korea
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- Ramasamy Paulmurugan
- Department of Radiology Molecular Imaging Program at Stanford Stanford University School of Medicine Stanford University Palo Alto CA 94304 USA
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- Woong Kyo Jeong
- Department of Orthopedic Surgery Korea University Anam Hospital Seoul 02841 Republic of Korea
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- Ki‐Bum Lee
- Department of Chemistry and Chemical Biology Rutgers University Piscataway NJ 08854 USA
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- Taeghwan Hyeon
- Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826 Republic of Korea
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- Dokyoon Kim
- Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 08826 Republic of Korea
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- Heemin Kang
- Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea
抄録
<jats:title>Abstract</jats:title><jats:p>In native microenvironment, diverse physical barriers exist to dynamically modulate stem cell recruitment and differentiation for tissue repair. In this study, nanoassembly‐based magnetic screens of various sizes are utilized, and they are elastically tethered over an RGD ligand (cell‐adhesive motif)‐presenting material surface to generate various nanogaps between the screens and the RGDs without modulating the RGD density. Large screens exhibiting low RGD distribution stimulate integrin clustering to facilitate focal adhesion, mechanotransduction, and differentiation of stem cells, which are not observed with small screens. Magnetic downward pulling of the large screens decreases the nanogaps, which dynamically suppress the focal adhesion, mechanotransduction, and differentiation of stem cells. Conversely, magnetic upward pulling of the small screens increases the nanogaps, which dynamically activates focal adhesion, mechanotransduction, and differentiation of stem cells. This regulation mechanism is also shown to be effective in the microenvironment in vivo. Further diversifying the geometries of the physical screens can further enable diverse modalities of multifaceted and safe unscreening of the distributed RGDs to unravel and modulate stem cell differentiation for tissue repair.</jats:p>
収録刊行物
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- Advanced Materials
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Advanced Materials 34 (2), 2021-11-14
Wiley