Infrared molecular fingerprinting of blood-based liquid biopsies for the detection of cancer

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  • Marinus Huber
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics
  • Kosmas V Kepesidis
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics
  • Liudmila Voronina
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics
  • Frank Fleischmann
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics
  • Ernst Fill
    Max Planck Institute of Quantum Optics (MPQ), Laboratory for Attosecond Physics
  • Jacqueline Hermann
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics
  • Ina Koch
    Asklepios Biobank for Lung Diseases, Department of Thoracic Surgery, Member of the German Center for Lung Research, DZL, Asklepios Fachkliniken München-Gauting
  • Katrin Milger-Kneidinger
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Internal Medicine V
  • Thomas Kolben
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Obstetrics and Gynecology, Breast Center and Comprehensive Cancer Center (CCLMU)
  • Gerald B Schulz
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Urology
  • Friedrich Jokisch
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Urology
  • Jürgen Behr
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Internal Medicine V
  • Nadia Harbeck
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Obstetrics and Gynecology, Breast Center and Comprehensive Cancer Center (CCLMU)
  • Maximilian Reiser
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Clinical Radiology
  • Christian Stief
    University Hospital of the Ludwig Maximilians University Munich (LMU), Department of Urology
  • Ferenc Krausz
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics
  • Mihaela Zigman
    Ludwig Maximilians University Munich (LMU), Department of Laser Physics

Description

<jats:p>Recent omics analyses of human biofluids provide opportunities to probe selected species of biomolecules for disease diagnostics. Fourier-transform infrared (FTIR) spectroscopy investigates the full repertoire of molecular species within a sample at once. Here, we present a multi-institutional study in which we analysed infrared fingerprints of plasma and serum samples from 1639 individuals with different solid tumours and carefully matched symptomatic and non-symptomatic reference individuals. Focusing on breast, bladder, prostate, and lung cancer, we find that infrared molecular fingerprinting is capable of detecting cancer: training a support vector machine algorithm allowed us to obtain binary classification performance in the range of 0.78–0.89 (area under the receiver operating characteristic curve [AUC]), with a clear correlation between AUC and tumour load. Intriguingly, we find that the spectral signatures differ between different cancer types. This study lays the foundation for high-throughput onco-IR-phenotyping of four common cancers, providing a cost-effective, complementary analytical tool for disease recognition.</jats:p>

Journal

  • eLife

    eLife 10 e68758-, 2021-10-26

    eLife Sciences Publications, Ltd

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