<i>ToxTrac</i>: A fast and robust software for tracking organisms

<jats:title>Abstract</jats:title><jats:p> <jats:list> <jats:list-item><jats:p>Behavioural analysis based on video recording is becoming increasingly popular within research fields such as; ecology, medicine, ecotoxicology and toxicology. However, the programs available to analyse the data, which are free of cost, user‐friendly, versatile, robust, fast and provide reliable statistics for different organisms (invertebrates, vertebrates and mammals) are significantly limited.</jats:p></jats:list-item> <jats:list-item><jats:p>We present an automated open‐source executable software (<jats:italic>ToxTrac</jats:italic>) for image‐based tracking that can simultaneously handle several organisms monitored in a laboratory environment. We compare the performance of <jats:italic>ToxTrac</jats:italic> with current accessible programs on the web.</jats:p></jats:list-item> <jats:list-item><jats:p>The main advantages of <jats:italic>ToxTrac</jats:italic> are as follows: (i) no specific knowledge of the geometry of the tracked bodies is needed; (ii) processing speed, <jats:italic>ToxTrac</jats:italic> can operate at a rate >25 frames per second in <jats:styled-content style="fixed-case">HD</jats:styled-content> videos using modern computers; (iii) simultaneous tracking of multiple organisms in multiple arenas; (iv) integrated distortion correction and camera calibration; (v) robust against false positives; (vi) preservation of individual identification; (vii) useful statistics and heat maps in real scale are exported in image, text and excel formats.</jats:p></jats:list-item> <jats:list-item><jats:p><jats:italic>ToxTrac</jats:italic> can be used for high speed tracking of insects, fish, rodents or other species, and provides useful locomotor information in animal behavior experiments. Download <jats:italic>ToxTrac</jats:italic> here: <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="https://toxtrac.sourceforge.io">https://toxtrac.sourceforge.io</jats:ext-link> (Current version v2.61).</jats:p></jats:list-item> </jats:list> </jats:p>