A visual opsin from jellyfish enables precise temporal control of G protein signalling
抄録
<jats:title>Abstract</jats:title><jats:p>Phototransduction is mediated by distinct types of G protein cascades in different animal taxa: bilateral invertebrates typically utilise the Gαq pathway whereas vertebrates typically utilise the Gαt(i/o) pathway. By contrast, photoreceptors in jellyfish (Cnidaria) utilise the Gαs intracellular pathway, similar to olfactory transduction in mammals<jats:sup>1</jats:sup>. How this habitually slow pathway has adapted to support dynamic vision in jellyfish remains unknown. Here we study a light-sensing protein (rhodopsin) from the box jellyfish <jats:italic>Carybdea rastonii</jats:italic> and uncover a mechanism that dramatically speeds up phototransduction: an uninterrupted G protein-coupled receptor – G protein complex. Unlike known G protein-coupled receptors (GPCRs), this rhodopsin constitutively binds a single downstream Gαs partner to enable G-protein activation and inactivation within tens of milliseconds. We use this GPCR in a viral gene therapy to restore light responses in blind mice.</jats:p>
収録刊行物
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- Nature Communications
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Nature Communications 14 (1), 2023-04-28
Springer Science and Business Media LLC