Photo‐Degradable Protein‐Polymer Hybrid Shells for Caging Living Cells
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- Satoshi Yamaguchi
- Research Center for Advanced Science and Technology The University of Tokyo 4-6-1 Komaba, Meguro-ku Tokyo 153–8904 Japan
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- Kazuki Chujo
- Department of Chemistry and Biotechnology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113–8656 Japan
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- Noriyuki Ohashi
- Department of Chemistry and Biotechnology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113–8656 Japan
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- Kosuke Minamihata
- Department of Applied Chemistry Graduate School of Engineering Kyushu University 744 Moto-oka Fukuoka 819–0395 Japan
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- Teruyuki Nagamune
- Department of Chemistry and Biotechnology The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113–8656 Japan
書誌事項
- 公開日
- 2022-02-03
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#am
- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/chem.202103941
- 公開者
- Wiley
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説明
<jats:title>Abstract</jats:title><jats:p>There is growing demand for the precise remote control of cellular functions in various fields. Herein, a method for caging mammalian cells by coating with photodegradable protein‐polymer hybrid shells to photo‐control their functions without genetic engineering is reported. A layer‐by‐layer assembly of photocleavable synthetic materials through biotin‐streptavidin (SA) binding was employed for cell coating. The cell surfaces were first biotinylated with photocleavable biotinylated poly(ethylene glycol)(PEG)‐lipid and then coated by repeatedly layering SA and micelles of the PEG‐lipid and photocleavable biotinylated four‐arm PEG. The cell extension and adhesion were suppressed with the shells and then triggered with the degradation of the shells by light exposure. Macrophage phagocytosis was also stopped by caging with the shells and restarted by light‐guided uncaging. This study provides the first proof of principle that cellular functions can be remotely controlled by steric hinderance of cell surfaces with photodegradable materials.</jats:p>
収録刊行物
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- Chemistry – A European Journal
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Chemistry – A European Journal 28 (12), 2022-02-03
Wiley
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詳細情報 詳細情報について
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- CRID
- 1360021390553982592
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- ISSN
- 15213765
- 09476539
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- PubMed
- 35037703
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- Web Site
- https://onlinelibrary.wiley.com/doi/pdf/10.1002/chem.202103941
- https://onlinelibrary.wiley.com/doi/full-xml/10.1002/chem.202103941
- https://chemistry-europe.onlinelibrary.wiley.com/doi/am-pdf/10.1002/chem.202103941
- https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/chem.202103941
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- 資料種別
- journal article
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- データソース種別
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- KAKEN
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