Osteogenic capacity of octacalcium phosphate involving macrophage polarization
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- Linghao Xiao
- Division of Craniofacial Function Engineering Tohoku University Graduate School of Dentistry Sendai Japan
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- Yukari Shiwaku
- Division of Craniofacial Function Engineering Tohoku University Graduate School of Dentistry Sendai Japan
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- Ryo Hamai
- Division of Craniofacial Function Engineering Tohoku University Graduate School of Dentistry Sendai Japan
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- Kazuyoshi Baba
- Department of Orthopedic Surgery Tohoku University Graduate School of Medicine Sendai Japan
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- Kaori Tsuchiya
- Division of Craniofacial Function Engineering Tohoku University Graduate School of Dentistry Sendai Japan
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- Satoshi Imazato
- Department of Biomaterials Science Osaka University Graduate School of Dentistry Suita Japan
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- Osamu Suzuki
- Division of Craniofacial Function Engineering Tohoku University Graduate School of Dentistry Sendai Japan
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- Keiichi Sasaki
- Division of Advanced Prosthetic Dentistry Tohoku University Graduate School of Dentistry Sendai Japan
書誌事項
- 公開日
- 2022-12-27
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/jbm.a.37484
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>Previous research has found that octacalcium phosphate (OCP) increases macrophage accumulation and alters the initial inflammatory response. However, the role of the immune response induced by OCP in osteogenesis remains unknown. This study investigated the behavior of macrophages and bone regeneration capacity during the early inflammatory stage of OCP‐mediated osteogenesis. To assess the change in macrophage polarization and osteogenic capacity, we used a standardized rat defect model filled with OCP or calcium‐deficient hydroxyapatite (CDHA)—a material obtained through the hydrolysis of the original OCP. OCP or CDHA granules were incubated with RAW264 cells for 5 days to investigate the effect of physicochemical characteristics on macrophage cytokine/chemokine expression in vitro. Our in vivo results show that due to the OCP implantation, macrophages in the rat tibial defect area tend to polarize to the M2 phenotype (anti‐inflammatory) and inhibit the formation of the M1 phenotype (pro‐inflammatory). In comparison to CDHA, OCP exhibited superior bone regeneration potential due to its rapid promotion of cortical bone healing and stimulation of macrophage‐related growth factors. Furthermore, our in vitro results have shown that OCP regulates the expression of macrophage chemokines over time. Compared to incubation with CDHA, incubation with OCP caused changes in the ionic microenvironment. These findings suggest that the OCP‐mediated macrophage polarization and secretion profile not only regulate immune function but also positively affect osteogenesis.</jats:p>
収録刊行物
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- Journal of Biomedical Materials Research Part A
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Journal of Biomedical Materials Research Part A 111 (7), 1006-1020, 2022-12-27
Wiley
