De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
書誌事項
- 公開日
- 2022-07-28
- 権利情報
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- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
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- 10.1038/s41467-022-31746-x
- 公開者
- Springer Science and Business Media LLC
説明
<jats:title>Abstract</jats:title> <jats:p> Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal <jats:italic>Bacillus thuringiensis</jats:italic> subsp. <jats:italic>israelensis</jats:italic> and <jats:italic>jegathesan</jats:italic> , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined <jats:italic>de novo</jats:italic> using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control. </jats:p>
収録刊行物
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- Nature Communications
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Nature Communications 13 (1), 4376-, 2022-07-28
Springer Science and Business Media LLC