Preventive Inositol Hexaphosphate Extracted from Rice Bran Inhibits Colorectal Cancer through Involvement of Wnt/<b><i>β</i></b>-Catenin and COX-2 Pathways
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- Nurul Husna Shafie
- Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
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- Norhaizan Mohd Esa
- Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
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- Hairuszah Ithnin
- UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
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- Abdah Md Akim
- Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
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- Norazalina Saad
- UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
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- Ashok Kumar Pandurangan
- Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
説明
<jats:p>Nutritional or dietary factors have drawn attention due to their potential as an effective chemopreventive agent, which is considered a more rational strategy in cancer treatment. This study was designed to evaluate the effect of IP<jats:sub>6</jats:sub>extracted from rice bran on azoxymethane- (AOM-) induced colorectal cancer (CRC) in rats. Initially, male Sprague Dawley rats were divided into 5 groups, with 6 rats in each group. The rats received two intraperitoneal (<jats:italic>i.p.</jats:italic>) injections of AOM in saline (15 mg/kg body weight) over a 2-week period to induce CRC. IP<jats:sub>6</jats:sub>was given in three concentrations, 0.2% (w/v), 0.5% (w/v), and 1.0% (w/v), via drinking water for 16 weeks. The deregulation of the Wnt/<jats:italic>β</jats:italic>-catenin signaling pathway and the expression of cyclooxygenase (COX)-2 have been implicated in colorectal tumorigenesis.<jats:italic>β</jats:italic>-Catenin and COX-2 expressions were analysed using the quantitative RT-PCR and Western blotting. Herein, we reported that the administration of IP<jats:sub>6</jats:sub>markedly suppressed the incidence of tumors when compared to the control. Interestingly, the administration of IP<jats:sub>6</jats:sub>had also markedly decreased<jats:italic>β</jats:italic>-catenin and COX-2 in colon tumors. Thus, the downregulation of<jats:italic>β</jats:italic>-catenin and COX-2 could play a role in inhibiting the CRC development induced by IP<jats:sub>6</jats:sub>and thereby act as a potent anticancer agent.</jats:p>
収録刊行物
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- BioMed Research International
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BioMed Research International 2013 1-10, 2013
Hindawi Limited
