Quantitative assessment of eye phenotypes for functional genetic studies using<i>Drosophila melanogaster</i>

DOI DOI HANDLE PDF Web Site ほか1件をすべて表示 一部だけ表示 研究データあり 被引用文献9件 参考文献60件 オープンアクセス

説明

<jats:title>Abstract</jats:title><jats:p>About two-thirds of the vital genes in the<jats:italic>Drosophila</jats:italic>genome are involved in eye development, making the fly eye an excellent genetic system to study cellular function and development, neurodevelopment/degeneration, and complex diseases such as cancer and diabetes. We developed a novel computational method, implemented as Flynotyper software (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://flynotyper.sourceforge.net">http://flynotyper.sourceforge.net</jats:ext-link>), to quantitatively assess the morphological defects in the<jats:italic>Drosophila</jats:italic>eye resulting from genetic alterations affecting basic cellular and developmental processes. Flynotyper utilizes a series of image processing operations to automatically detect the fly eye and the individual ommatidium, and calculates a phenotypic score as a measure of the disorderliness of ommatidial arrangement in the fly eye. As a proof of principle, we tested our method by analyzing the defects due to eye-specific knockdown of<jats:italic>Drosophila</jats:italic>orthologs of 12 neurodevelopmental genes to accurately document differential sensitivities of these genes to dosage alteration. We also evaluated eye images from six independent studies assessing the effect of overexpression of repeats, candidates from peptide library screens, and modifiers of neurotoxicity and developmental processes on eye morphology, and show strong concordance with the original assessment. We further demonstrate the utility of this method by analyzing 16 modifiers of<jats:italic>sine oculis</jats:italic>obtained from two genome-wide deficiency screens of<jats:italic>Drosophila</jats:italic>and accurately quantifying the effect of its enhancers and suppressors during eye development. Our method will complement existing assays for eye phenotypes and increase the accuracy of studies that use fly eyes for functional evaluation of genes and genetic interactions.</jats:p>

収録刊行物

被引用文献 (9)*注記

もっと見る

参考文献 (60)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ