Enzymatic Bioelectrocatalysis for Enzymology Applications

Matthew J. Kummer, Shelley D. Minteer

doi:10.1002/celc.202000239

Enzymatic Bioelectrocatalysis for Enzymology Applications

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Matthew J. Kummer

Department of Chemistry University of Utah 315 S 1400 E Salt Lake City UT 84112 USA
Shelley D. Minteer

Department of Chemistry University of Utah 315 S 1400 E Salt Lake City UT 84112 USA

書誌事項

公開日: 2020-04-06

権利情報

http://onlinelibrary.wiley.com/termsAndConditions#am
http://onlinelibrary.wiley.com/termsAndConditions#vor

DOI

10.1002/celc.202000239

公開者: Wiley

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説明

<jats:title>Abstract</jats:title><jats:p>Enzymatic biosensors are commonly used to evaluate the concentration of individual analytes in samples (e. g., glucose in blood, lactate in sweat, etc.), where the working electrode utilizes an enzyme (a “bioelectrode”) as the chemically selective agent for electrochemical analyses. However, those same enzymatic bioelectrodes can be used for the analytical characterization of enzymes as a new biophysical characterization tool for enzymologists. This Minireview will detail recent work focused on utilizing enzymatic bioelectrocataysis for enzymology applications. Specifically, it will talk about the use of enzymatic bioelectrodes for studying kinetics, inhibition, transport, and thermodynamics. It will also compare and contrast electrochemical techniques with spectrophotometric techniques for enzymology.</jats:p>