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- G. K. Bissonnette
- Department of Microbiology, Montana State University, Bozeman, Montana 59715
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- J. J. Jezeski
- Department of Microbiology, Montana State University, Bozeman, Montana 59715
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- G. A. McFeters
- Department of Microbiology, Montana State University, Bozeman, Montana 59715
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- D. G. Stuart
- Department of Microbiology, Montana State University, Bozeman, Montana 59715
書誌事項
- 公開日
- 1977-03
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/aem.33.3.590-595.1977
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:p> Various recovery methods used to detect coliforms in water were evaluated by applying the membrane filter chamber technique. The membrane filter chambers, containing pure-culture suspensions of <jats:italic>Escherichia coli</jats:italic> or natural suspensions of raw sewage, were immersed in the stream environment. Samples were withdrawn from the chamber at regular time intervals and enumerated by several detection methods. In general, multiple-tube fermentation techniques gave better recovery than plating or membrane filtration procedures. The least efficient method of recovery resulted when using membrane filtration procedures, especially as the exposure period of the organisms to the stream environment increased. A 2-h enrichment on a rich, nonselective medium before exposure to selective media improved the recovery of fecal coliforms with membrane filtration techniques. Substantially enhanced recoveries of <jats:italic>E. coli</jats:italic> from pure-culture suspensions and of fecal coliforms from raw-sewage suspensions were observed when compared with recoveries obtained by direct primary exposure to selective media. Such an enrichment period appears to provide a nontoxic environment for the gradual adjustment and repair of injured cells. </jats:p>
収録刊行物
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- Applied and Environmental Microbiology
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Applied and Environmental Microbiology 33 (3), 590-595, 1977-03
American Society for Microbiology
