Chromium reduction in Pseudomonas putida

  • Y Ishibashi
    Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680.
  • C Cervantes
    Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680.
  • S Silver
    Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680.

書誌事項

公開日
1990-07
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/aem.56.7.2268-2270.1990
公開者
American Society for Microbiology

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説明

<jats:p>Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.</jats:p>

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