Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals
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- Antal Berényi
- New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
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- Zoltán Somogyvári
- Center for Molecular and Behavioral Neuroscience, Rutgers, The State University of New Jersey, Newark, New Jersey;
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- Anett J. Nagy
- MTA-SZTE “Momentum” Oscillatory Neuronal Networks Research Group, Department of Physiology, University of Szeged, Szeged, Hungary;
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- Lisa Roux
- New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
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- John D. Long
- New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
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- Shigeyoshi Fujisawa
- New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
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- Eran Stark
- New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
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- Anthony Leonardo
- Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia
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- Timothy D. Harris
- Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia
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- György Buzsáki
- New York University Neuroscience Institute, School of Medicine, New York University, New York, New York;
抄録
<jats:p> Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function. </jats:p>
収録刊行物
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- Journal of Neurophysiology
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Journal of Neurophysiology 111 (5), 1132-1149, 2014-03-01
American Physiological Society