Serotonin-2C receptor involved serotonin-induced Ca2+ mobilisations in neuronal progenitors and neurons in rat suprachiasmatic nucleus
説明
<jats:title>Abstract</jats:title><jats:p>The hypothalamic suprachiasmatic nucleus (SCN), the central circadian pacemaker in mammals, undergoes serotonergic regulation, but the underlying mechanisms remain obscure. Here, we generated a subclone of an SCN progenitor cell line expressing Ca<jats:sup>2+</jats:sup> sensors (SCN2.2YC) and compared its 5-HT receptor signalling with that of rat SCN neurons in brain slices. SCN2.2YC cells expressed 5-HT1A/2A/2B/2C, but not 5A/7, while all six subtypes were expressed in SCN tissues. High K<jats:sup>+</jats:sup> or 5-HT increased cytosolic Ca<jats:sup>2+</jats:sup> in SCN2.2YC cells. The 5-HT responses were inhibited by ritanserin and SB-221284, but resistant to WAY-100635 and RS-127445, suggesting predominant involvement of 5-HT2C for Ca<jats:sup>2+</jats:sup> mobilisations. Consistently, Ca<jats:sup>2+</jats:sup> imaging and voltage-clamp electrophysiology using rat SCN slices demonstrated post-synaptic 5-HT2C expression. Because 5-HT2C expression was postnatally increased in the SCN and 5-HT-induced Ca<jats:sup>2+</jats:sup> mobilisations were amplified in differentiated SCN2.2YC cells and developed SCN neurons, we suggest that this signalling development occurs in accordance with central clock maturations.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 4 (1), 4106-, 2014-02-17
Springer Science and Business Media LLC
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詳細情報 詳細情報について
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- CRID
- 1360283691832082688
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- ISSN
- 20452322
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
