Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice

DOI Web Site 被引用文献41件 参考文献24件
  • Satoru Ishikawa
    Soil Environment Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan;
  • Yasuhiro Ishimaru
    Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan;
  • Masato Igura
    Soil Environment Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan;
  • Masato Kuramata
    Soil Environment Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan;
  • Tadashi Abe
    Soil Environment Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan;
  • Takeshi Senoura
    Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan;
  • Yoshihiro Hase
    Quantum Beam Science Directorate, Japan Atomic Energy Agency, Takasaki, Gunma 370-1292, Japan;
  • Tomohito Arao
    Soil Environment Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan;
  • Naoko K. Nishizawa
    Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan;
  • Hiromi Nakanishi
    Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan;

抄録

<jats:p> Rice ( <jats:italic>Oryza sativa</jats:italic> L.) grain is a major dietary source of cadmium (Cd), which is toxic to humans, but no practical technique exists to substantially reduce Cd contamination. Carbon ion-beam irradiation produced three rice mutants with <0.05 mg Cd⋅kg <jats:sup>−1</jats:sup> in the grain compared with a mean of 1.73 mg Cd⋅kg <jats:sup>−1</jats:sup> in the parent, Koshihikari. We identified the gene responsible for reduced Cd uptake and developed a strategy for marker-assisted selection of low-Cd cultivars. Sequence analysis revealed that these mutants have different mutations of the same gene ( <jats:italic>OsNRAMP5</jats:italic> ), which encodes a natural resistance-associated macrophage protein. Functional analysis revealed that the defective transporter protein encoded by the mutant <jats:italic>osnramp5</jats:italic> greatly decreases Cd uptake by roots, resulting in decreased Cd in the straw and grain. In addition, we developed DNA markers to facilitate marker-assisted selection of cultivars carrying <jats:italic>osnramp5</jats:italic> . When grown in Cd-contaminated paddy fields, the mutants have nearly undetectable Cd in their grains and exhibit no agriculturally or economically adverse traits. Because mutants produced by ion-beam radiation are not transgenic plants, they are likely to be accepted by consumers and thus represent a practical choice for rice production worldwide. </jats:p>

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