Sox10- Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes

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<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>While several mouse strains have recently been developed for tracing neural crest or oligodendrocyte lineages, each strain has inherent limitations. The connection between human<jats:italic>SOX10</jats:italic>mutations and neural crest cell pathogenesis led us to focus on the<jats:italic>Sox10</jats:italic>gene, which is critical for neural crest development. We generated<jats:italic>Sox10-</jats:italic>Venus BAC transgenic mice to monitor Sox10 expression in both normal development and in pathological processes.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Tissue fluorescence distinguished neural crest progeny cells and oligodendrocytes in the<jats:italic>Sox10-</jats:italic>Venus mouse embryo. Immunohistochemical analysis confirmed that Venus expression was restricted to cells expressing endogenous Sox10. Time-lapse imaging of various tissues in<jats:italic>Sox10-</jats:italic>Venus mice demonstrated that Venus expression could be visualized at the single-cell level<jats:italic>in vivo</jats:italic>due to the intense, focused Venus fluorescence. In the adult<jats:italic>Sox10-</jats:italic>Venus mouse, several types of mature and immature oligodendrocytes along with Schwann cells were clearly labeled with Venus, both before and after spinal cord injury.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In the newly-developed<jats:italic>Sox10-</jats:italic>Venus transgenic mouse, Venus fluorescence faithfully mirrors endogenous Sox10 expression and allows for<jats:italic>in vivo</jats:italic>imaging of live cells at the single-cell level. This<jats:italic>Sox10-</jats:italic>Venus mouse will thus be a useful tool for studying neural crest cells or oligodendrocytes, both in development and in pathological processes.</jats:p></jats:sec>

収録刊行物

  • Molecular Brain

    Molecular Brain 3 (1), 31-, 2010-10-31

    Springer Science and Business Media LLC

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