Sox10- Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes
説明
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>While several mouse strains have recently been developed for tracing neural crest or oligodendrocyte lineages, each strain has inherent limitations. The connection between human<jats:italic>SOX10</jats:italic>mutations and neural crest cell pathogenesis led us to focus on the<jats:italic>Sox10</jats:italic>gene, which is critical for neural crest development. We generated<jats:italic>Sox10-</jats:italic>Venus BAC transgenic mice to monitor Sox10 expression in both normal development and in pathological processes.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Tissue fluorescence distinguished neural crest progeny cells and oligodendrocytes in the<jats:italic>Sox10-</jats:italic>Venus mouse embryo. Immunohistochemical analysis confirmed that Venus expression was restricted to cells expressing endogenous Sox10. Time-lapse imaging of various tissues in<jats:italic>Sox10-</jats:italic>Venus mice demonstrated that Venus expression could be visualized at the single-cell level<jats:italic>in vivo</jats:italic>due to the intense, focused Venus fluorescence. In the adult<jats:italic>Sox10-</jats:italic>Venus mouse, several types of mature and immature oligodendrocytes along with Schwann cells were clearly labeled with Venus, both before and after spinal cord injury.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>In the newly-developed<jats:italic>Sox10-</jats:italic>Venus transgenic mouse, Venus fluorescence faithfully mirrors endogenous Sox10 expression and allows for<jats:italic>in vivo</jats:italic>imaging of live cells at the single-cell level. This<jats:italic>Sox10-</jats:italic>Venus mouse will thus be a useful tool for studying neural crest cells or oligodendrocytes, both in development and in pathological processes.</jats:p></jats:sec>
収録刊行物
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- Molecular Brain
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Molecular Brain 3 (1), 31-, 2010-10-31
Springer Science and Business Media LLC
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キーワード
- Mice, Transgenic
- Time-Lapse Imaging
- Cellular and Molecular Neuroscience
- Mice
- Bacterial Proteins
- Genes, Reporter
- Animals
- Humans
- Cell Lineage
- RC346-429
- Molecular Biology
- Spinal Cord Injuries
- Staining and Labeling
- SOXE Transcription Factors
- Research
- Embryo, Mammalian
- Mice, Inbred C57BL
- Luminescent Proteins
- Oligodendroglia
- Neural Crest
- Female
- Neurology. Diseases of the nervous system
詳細情報 詳細情報について
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- CRID
- 1360283693957794560
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- ISSN
- 17566606
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- PubMed
- 21034515
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE