Frequent occurrence of diploid a or α sporogenous mater cells and construction of ploidy series in the sake yeast kyokai no. 9

Yoshinobu Tani, Yukio Tomohiro, Akira Miyata, Shinsaku Hayashida

doi:10.1016/0922-338x(90)90044-w

Frequent occurrence of diploid a or α sporogenous mater cells and construction of ploidy series in the sake yeast kyokai no. 9

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Abstract Diploid a or α sporogenous mater cells were frequently obtained in the sake yeast Kyokai no. 9 by heat treatment followed by random spore plating. Polyploid cells were constructed between the opposite mater cells by the mass mating technique, followed by the isolation of the zygotes using a micromanipulator.