Establishment of a short‐term, <i>in vivo</i> screening method for detecting chemicals with juvenile hormone activity using adult <i>Daphnia magna</i>

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  • Ryoko Abe
    Graduate School of Frontier Sciences The University of Tokyo 5‐1‐5 Kashiwanoha Kashiwa Chiba 277‐8561 Japan
  • Haruna Watanabe
    Environmental Quality Measurement Section, Research Center for Environmental Risk National Institute for Environmental Studies 16‐2 Onogawa Tsukuba Ibaraki 305‐8506 Japan
  • Masumi Yamamuro
    Graduate School of Frontier Sciences The University of Tokyo 5‐1‐5 Kashiwanoha Kashiwa Chiba 277‐8561 Japan
  • Taisen Iguchi
    Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, and Department of Basic Biology, School of Life Science Graduate University for Advanced Studies (SOKENDAI) 5‐1 Higashiyama, Myodaiji Okazaki Aichi 444‐8787 Japan
  • Norihisa Tatarazako
    Graduate School of Frontier Sciences The University of Tokyo 5‐1‐5 Kashiwanoha Kashiwa Chiba 277‐8561 Japan

Description

<jats:title>ABSTRACT</jats:title><jats:p>Juvenile hormone (JH) and JH agonists have been shown to induce male offspring production in various daphnids, including <jats:italic>Daphnia magna</jats:italic> using OECD TG211. The critical period (about 1h) for JH action on ova in the parent's ovary to induce male offspring is existing at 7‐8h later from ovulation. Therefore, we considered that adult <jats:italic>D</jats:italic>. <jats:italic>magna</jats:italic> could be used to produce a short‐term screening method for detecting JH analogs. Using this method, we successfully demonstrated male offspring induction in the second broods after exposure to JH or JH agonists. After investigating the exposure time, the number of repetitions and the exposure concentration, we established a short‐term, <jats:italic>in vivo</jats:italic> screening method for detecting JH analogs using adult <jats:italic>D</jats:italic>. <jats:italic>magna</jats:italic>. We examined positive and negative control chemicals using a previously developed method and verified the validity of our new testing method. Copyright © 2014 John Wiley & Sons, Ltd.</jats:p>

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