Mn‐bicine: A low affinity chelate for manganese ion enhanced MRI
書誌事項
- 公開日
- 2010-11-04
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/mrm.22680
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>The toxicity of free Mn<jats:sup>2+</jats:sup> is a bottleneck for the in vivo application of manganese ion enhanced MRI. To reduce free Mn<jats:sup>2+</jats:sup> concentration ([Mn<jats:sup>2+</jats:sup>]), a low affinity chelate reagent: <jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>‐bis(2‐hydroxyethyl)glycine (bicine) was used. Considering the conditional association constant of Mn‐bicine at pH 7.4 (10<jats:sup>2.9</jats:sup> M<jats:sup>−1</jats:sup>), (i) a 100 mM Mn‐bicine solution should contain about 10 mM of free manganese ion, but (ii) free manganese will make up 3/4 of the final plasma concentration (0.5 mM) with an intravenous infusion of 100 mM Mn‐bicine. The <jats:italic>T</jats:italic><jats:sub>1</jats:sub> relaxivity of Mn‐bicine in a 5 mM Mn‐bicine solution was estimated as 5 mM<jats:sup>−1</jats:sup> sec<jats:sup>−1</jats:sup> at 24°C, 7 T in a pH range of 6.8–7.5. Mn‐bicine demonstrated a tendency for better contractility when employed with an isolated perfused frog heart, compared with MnCl<jats:sub>2</jats:sub>. A venous infusion of 100 mM Mn‐bicine (8.3 μmol kg<jats:sup>−1</jats:sup> min<jats:sup>−1</jats:sup>) showed a minimal decrease and maintained a constant heart rate level and arterial pressure in rats, while rats infused with 100 mM of MnCl<jats:sub>2</jats:sub> showed a significant suppression of the hemodynamic functions. Thus, Mn‐bicine appears to be a better choice for maintaining the vital conditions of experimental animals, and may improve the reproducibility of manganese ion enhanced MRI. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.</jats:p>
収録刊行物
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- Magnetic Resonance in Medicine
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Magnetic Resonance in Medicine 65 (4), 1005-1012, 2010-11-04
Wiley
