Promotion of skeletal muscle repair in a rat skeletal muscle injury model by local injection of human adipose tissue-derived regenerative cells

  • Ryo Mori
    Department of Orthopedic Surgery, Graduate School of Biomedical Sciences; Hiroshima University; Japan
  • Naosuke Kamei
    Department of Orthopedic Surgery, Graduate School of Biomedical Sciences; Hiroshima University; Japan
  • Shingo Okawa
    Department of Orthopedic Surgery, Graduate School of Biomedical Sciences; Hiroshima University; Japan
  • Akihiro Nakabayashi
    Department of Orthopedic Surgery, Graduate School of Biomedical Sciences; Hiroshima University; Japan
  • Kazunori Yokota
    Department of Plastic and Reconstructive Surgery, Graduate School of Biomedical Sciences; Hiroshima University; Japan
  • Yukihito Higashi
    Division of Regeneration and Medicine; Hiroshima University Hospital; Japan
  • Mitsuo Ochi
    Department of Orthopedic Surgery, Graduate School of Biomedical Sciences; Hiroshima University; Japan

Bibliographic Information

Other Title
  • Promotion of skeletal muscle repair by ADRC injection
Published
2012-12-14
Resource Type
journal article
Rights Information
  • http://doi.wiley.com/10.1002/tdm_license_1.1
DOI
  • 10.1002/term.1659
Publisher
Hindawi Limited

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Description

Human adipose tissue-derived regenerative cells (ADRCs) can be isolated easily and aseptically from unwanted subcutaneous fat without culturing. ADRCs have been used in clinical cosmetic therapy. In addition, they are expected to be an attractive and feasible source of cell-based therapies in regenerative medicine. Therefore, this paper investigates whether transplantation of human adult ADRCs into skeletal muscle injury models promotes the repair of muscle tissues. This was done by locally injecting human ADRCs into an injured site after laceration of the nude-rat tibialis anterior muscle. Phosphate-buffered saline (PBS) and bone marrow mononuclear cells (MNCs) were injected as negative and positive controls, respectively. After injury, recovery of muscle strength was accelerated by transplantation of ADRCs compared to administration of PBS and MNCs. Moreover, transplantation of ADRCs also enhanced angiogenesis and myogenesis, but the number of vascular and muscular cells labeled with the human cell-specific maker was limited at the injury site. Results showed that transplantation of ADRCs into a skeletal muscle injury model promoted repair of muscle tissues in a paracrine manner rather than differentiation of itself into blood vessels and myofibres. Thus, it is believed that ADRCs are a useful and feasible cell source not only for cosmetic therapy but also for regenerative therapy.

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