Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay

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Semaphorins, which were originally identified as axon-guidance molecules, also serve multiple functions in various phases of immune responses. In particular, several semaphorins regulate antigen presentation by dendritic cells (DCs) and resultant differentiation of CD4+ T cells. Here we describe a method for investigating both functions in vitro. This method might be used to obtain insight into novel aspects of semaphorin-mediated immunity.

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