Rapid and Reliable Method for Determining Irradiation Histories of Ground Beef and Prawns by Measuring 5,6-Dihydrothymidine

  • Naoki Fukui
    Division of Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Japan
  • Satoshi Takatori
    Division of Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Japan
  • Yoko Kitagawa
    Division of Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Japan
  • Takuya Fujiwara
    Division of Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Japan
  • Etsuko Ishikawa
    Laboratory of Quantum-Beam Chemistry and Biology, Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka, Japan
  • Takatomo Fujiyama
    Laboratory of Quantum-Beam Chemistry and Biology, Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka, Japan
  • Keiji Kajimura
    Division of Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Japan
  • Masakazu Furuta
    Laboratory of Quantum-Beam Chemistry and Biology, Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka, Japan
  • Hirotaka Obana
    Division of Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka, Japan

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説明

A rapid and reliable method for determining irradiation histories of ground beef and prawns was developed on the basis of a method for determining the irradiation history of beef liver by liquid chromatography-tandem mass spectrometry (LC-MS/MS) of 5,6-dihydrothymidine (DHdThd). Improvements in the method included the following: (1) 50% ethanol precipitation in the DNA extraction step was conducted before the RNase step, (2) snake venom phosphodiesterase I was used for DNA digestion to boost liberation of DHdThd, and (3) a matrix-matched calibration curve was used for determining DHdThd by LC-MS/MS analysis. This method successfully determined irradiation histories of ground beef and prawns. Furthermore, a close correlation between the formation of DHdThd and 2-alkylcyclobutanones, which are an established index of irradiation histories, was observed in ground beef. DHdThd in DNA could be a promising candidate for a new index of irradiation histories of various foods.

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