Adhesion properties of <i>Lactobacillus rhamnosus</i> mucus-binding factor to mucin and extracellular matrix proteins
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- Keita Nishiyama
- Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan
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- Koichi Nakamata
- Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan
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- Shintaro Ueno
- Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan
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- Akari Terao
- Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan
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- Ni Putu Desy Aryantini
- Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
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- I Nengah Sujaya
- Integrated Laboratory for Bioscience and Biotechnology, Udayana University, Bukit Jimbaran Campus, Badung, Bali, Indonesia
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- Kenji Fukuda
- Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
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- Tadasu Urashima
- Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
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- Yuji Yamamoto
- Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan
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- Takao Mukai
- Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan
書誌事項
- 公開日
- 2015-02-01
- 資源種別
- journal article
- 権利情報
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- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1080/09168451.2014.972325
- 公開者
- Informa UK Limited
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>We previously described potential probiotic Lactobacillus rhamnosus strains, isolated from fermented mare milk produced in Sumbawa Island, Indonesia, which showed high adhesion to porcine colonic mucin (PCM) and extracellular matrix (ECM) proteins. Recently, mucus-binding factor (MBF) was found in the GG strain of L. rhamnosus as a mucin-binding protein. In this study, we assessed the ability of recombinant MBF protein from the FSMM22 strain, one of the isolates of L. rhamnosus from fermented Sumbawa mare milk, to adhere to PCM and ECM proteins by overlay dot blot and Biacore assays. MBF bound to PCM, laminin, collagen IV, and fibronectin with submicromolar dissociation constants. Adhesion of the FSMM22 mbf mutant strain to PCM and ECM proteins was significantly less than that of the wild-type strain. Collectively, these results suggested that MBF contribute to L. rhamnosus host colonization via mucin and ECM protein binding.</jats:p>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 79 (2), 271-279, 2015-02-01
Informa UK Limited
