Application of local gene induction by infrared laser‐mediated microscope and temperature stimulator to amphibian regeneration study
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- Aiko Kawasumi‐Kita
- Department of Developmental Biology and Neurosciences Graduate School of Life Sciences Tohoku University Aramaki‐Aza‐Aoba 6‐3, Aoba‐ku Sendai Miyagi 980‐8578 Japan
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- Toshinori Hayashi
- School of Life Science Faculty of Medicine Tottori University Yonago Tottori 683‐8503 Japan
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- Takuya Kobayashi
- Department of Developmental Biology and Neurosciences Graduate School of Life Sciences Tohoku University Aramaki‐Aza‐Aoba 6‐3, Aoba‐ku Sendai Miyagi 980‐8578 Japan
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- Chikashi Nagayama
- Department of Developmental Biology and Neurosciences Graduate School of Life Sciences Tohoku University Aramaki‐Aza‐Aoba 6‐3, Aoba‐ku Sendai Miyagi 980‐8578 Japan
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- Shinichi Hayashi
- Department of Developmental Biology and Neurosciences Graduate School of Life Sciences Tohoku University Aramaki‐Aza‐Aoba 6‐3, Aoba‐ku Sendai Miyagi 980‐8578 Japan
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- Yasuhiro Kamei
- Spectrography and Bioimaging Facility National Institute for Basic Biology Myodaiji Okazaki Aichi 445‐8585 Japan
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- Yoshihiro Morishita
- Laboratory for Developmental Morphogeometry RIKEN Quantitative Biology Center Kobe Hyogo 650‐0047 Japan
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- Takashi Takeuchi
- School of Life Science Faculty of Medicine Tottori University Yonago Tottori 683‐8503 Japan
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- Koji Tamura
- Department of Developmental Biology and Neurosciences Graduate School of Life Sciences Tohoku University Aramaki‐Aza‐Aoba 6‐3, Aoba‐ku Sendai Miyagi 980‐8578 Japan
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- Hitoshi Yokoyama
- Department of Developmental Biology and Neurosciences Graduate School of Life Sciences Tohoku University Aramaki‐Aza‐Aoba 6‐3, Aoba‐ku Sendai Miyagi 980‐8578 Japan
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説明
<jats:p>Urodele amphibians (newts and salamanders) and anuran amphibians (frogs) are excellent research models to reveal mechanisms of three‐dimensional organ regeneration since they have exceptionally high regenerative capacity among tetrapods. However, the difficulty in manipulating gene expression in cells in a spatially restricted manner has so far hindered elucidation of the molecular mechanisms of organ regeneration in amphibians. Recently, local heat shock by laser irradiation has enabled local gene induction even at the single‐cell level in teleost fishes, nematodes, fruit flies and plants. In this study, local heat shock was made with infrared laser irradiation (<jats:styled-content style="fixed-case">IR</jats:styled-content>‐<jats:styled-content style="fixed-case">LEGO</jats:styled-content>) by using a gene expression inducible system in transgenic animals containing a heat shock promoter, and gene expression was successfully induced only in the target region of two amphibian species, <jats:italic>Xenopus laevis</jats:italic> and <jats:italic>Pleurodeles waltl</jats:italic> (a newt), at postembryonic stages. Furthermore, we induced spatially restricted but wider gene expression in <jats:italic>Xenopus laevis</jats:italic> tadpoles and froglets by applying local heat shock by a temperature‐controlled metal probe (temperature stimulator). The local gene manipulation systems, the <jats:styled-content style="fixed-case">IR</jats:styled-content>‐<jats:styled-content style="fixed-case">LEGO</jats:styled-content> and the temperature stimulator, enable us to do a rigorous cell lineage trace with the combination of the Cre‐LoxP system as well as to analyze gene function in a target region or cells with less off‐target effects in the study of amphibian regeneration.</jats:p>
収録刊行物
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- Development, Growth & Differentiation
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Development, Growth & Differentiation 57 (9), 601-613, 2015-10-29
Wiley
