Genome-Wide Analysis of Histone Modifications in Human Endometrial Stromal Cells
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- Isao Tamura
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Yasuyuki Ohkawa
- Department of Advanced Initiative Medicine (Y.O.), Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka, 812-8582, Japan;
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- Tetsuya Sato
- Division of Bioinformatics (T.S., M.S.), Medical Institute of Bioregulation, Kyushu University, Fukuoka, 812-8582, Japan
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- Mikita Suyama
- Division of Bioinformatics (T.S., M.S.), Medical Institute of Bioregulation, Kyushu University, Fukuoka, 812-8582, Japan
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- Kosuke Jozaki
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Maki Okada
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Lifa Lee
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Ryo Maekawa
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Hiromi Asada
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Shun Sato
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Yoshiaki Yamagata
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Hiroshi Tamura
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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- Norihiro Sugino
- Department of Obstetrics and Gynecology (I.T., K.J., M.O., L.L., R.M., H.A., S.S., Y.Y., H.T., N.S.), Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan;
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説明
<jats:p>Dramatic changes of gene expressions occur in human endometrial stromal cells (ESCs) during decidualization. The changes in gene expression are associated with changes of chromatin structure, which are regulated by histone modifications. Here we investigated genome-wide changes in histone modifications associated with decidualization in human ESCs using chromatin immunoprecipitation combined with next-generation sequencing. ESCs were incubated with estradiol and medroxyprogesterone acetate for 14 days to induce decidualization. The chromatin immunoprecipitation-sequence data showed that induction of decidualization increased H3K27ac and H3K4me3 signals in many genomic regions but decreased in only a few regions. Most of the H3K27ac-increased regions (80%) and half of the H3K4me3-increased regions were located in the distal promoter regions (more than 3 kb upstream or downstream of the transcription start site). RNA sequence showed that induction of decidualization up-regulated 881 genes, 223 of which had H3K27ac- or H3K4me3-increased regions in the proximal and distal promoter regions. Induction of decidualization increased the mRNA levels of these genes more than it increased the mRNA levels of genes without H3K27ac- or H3K4me3-increased regions. Pathway analysis revealed that up-regulated genes with the H3K27ac- or H3K4me3-increased regions were associated with the insulin signaling, which may be involved in glucose uptake that is necessary for ESCs to undergo decidualization. These results show that histone modification statuses on a genome-wide basis change in human ESCs during decidualization. The main changes of histone modifications are increases of H3K27ac and H3K4me3 in both the proximal and distal promoter regions, which are involved in the up-regulation of gene expression that occurs during decidualization.</jats:p>
収録刊行物
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- Molecular Endocrinology
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Molecular Endocrinology 28 (10), 1656-1669, 2014-10-01
The Endocrine Society
