Bulk <scp>RNA</scp> degradation by nitrogen starvation‐induced autophagy in yeast

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  • Hanghang Huang
    Department of Biotechnology Osaka University Suita Osaka Japan
  • Tomoko Kawamata
    Frontier Research Center Tokyo Institute of Technology Midori‐ku Yokohama Japan
  • Tetsuro Horie
    Frontier Research Center Tokyo Institute of Technology Midori‐ku Yokohama Japan
  • Hiroshi Tsugawa
    Department of Biotechnology Osaka University Suita Osaka Japan
  • Yasumune Nakayama
    Department of Biotechnology Osaka University Suita Osaka Japan
  • Yoshinori Ohsumi
    Frontier Research Center Tokyo Institute of Technology Midori‐ku Yokohama Japan
  • Eiichiro Fukusaki
    Department of Biotechnology Osaka University Suita Osaka Japan

説明

Autophagy is a catabolic process conserved among eukaryotes. Under nutrient starvation, a portion of the cytoplasm is non-selectively sequestered into autophagosomes. Consequently, ribosomes are delivered to the vacuole/lysosome for destruction, but the precise mechanism of autophagic RNA degradation and its physiological implications for cellular metabolism remain unknown. We characterized autophagy-dependent RNA catabolism using a combination of metabolome and molecular biological analyses in yeast. RNA delivered to the vacuole was processed by Rny1, a T2-type ribonuclease, generating 3'-NMPs that were immediately converted to nucleosides by the vacuolar non-specific phosphatase Pho8. In the cytoplasm, these nucleosides were broken down by the nucleosidases Pnp1 and Urh1. Most of the resultant bases were not re-assimilated, but excreted from the cell. Bulk non-selective autophagy causes drastic perturbation of metabolism, which must be minimized to maintain intracellular homeostasis.

収録刊行物

  • The EMBO Journal

    The EMBO Journal 34 (2), 154-168, 2014-12-02

    Springer Science and Business Media LLC

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参考文献 (49)*注記

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