Identification of RNA aptamer which specifically interacts with PtdIns(3)P
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説明
The phosphinositide PtdIns(3)P plays an important role in autophagy; however, the detailed mechanism of its activity remains unclear. Here, we used a Systematic Evolution of Ligands by EXponential enrichment (SELEX) screening approach to identify an RNA aptamer of 40 nucleotides that specifically recognizes and binds to intracellular lysosomal PtdIns(3)P. Binding occurs in a magnesium concentration- and pH-dependent manner, and consequently inhibits autophagy as determined by LC3II/I conversion, p62 degradation, formation of LC3 puncta, and lysosomal accumulation of Phafin2. These effects in turn inhibited lysosomal acidification, and the subsequent hydrolytic activity of cathepsin D following induction of autophagy. Given the essential role of PtdIns(3)P as a key targeting molecule for autophagy induction, identification of this novel PtdIns(3)P RNA aptamer provides new opportunities for investigating the biological functions and mechanisms of phosphoinositides.
収録刊行物
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- Biochemical and Biophysical Research Communications
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Biochemical and Biophysical Research Communications 517 (1), 146-154, 2019-09
Elsevier BV
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キーワード
詳細情報 詳細情報について
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- CRID
- 1360286991648354944
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- HANDLE
- 2115/75448
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- ISSN
- 0006291X
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- PubMed
- 31351587
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE