Specific Reconstruction on pRC Plasmid to Facilitate Its Universal Chromosomal Integration in Different <i>Ralstonia solanacearum</i> Species Complex Strains

DOI Web Site 被引用文献1件 参考文献12件
  • Yong Zhang
    College of Resources and Environment, Southwest University, Chongqing, China
  • Shengsheng Gao
    College of Resources and Environment, Southwest University, Chongqing, China
  • Peng Li
    Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Science, Hainan Normal University, China
  • Kouhei Ohnishi
    Research Institute of Molecular Genetics, Kochi University, Kochi, Japan

この論文をさがす

説明

<jats:p> The pRC system is an efficient tool for genetic studies in Ralstonia solanacearum, ensuring direct insertion of foreign gene elements into Ralstonia chromosome downstream of glms. This system is designed for double recombination across glms and the downstream region, which considerably simplifies genetic studies of complementation, overexpression, pathogenicity, and in-vivo promoter activity assays with monocopy in R. solanacearum, one of the most destructive plant-pathogenic bacteria worldwide. R. solanacearum is extremely heterogeneous and is currently referred to as a Ralstonia solanacearum species complex (RSSC). The glms gene is greatly conserved, but its downstream regions are mostly different in the RSSC, which limits the application of the current pRC plasmid in the RSSC. We compared all existing 132 genome sequences in a precise genomic glms downstream region and confirmed that the pRC system is appropriate for application of chromosomal integration in all RSSC strains but needs respective reconstruction on current pRC plasmids, since glms downstream regions are greatly variable in the RSSC. RSSC strains can be grouped according to identical glms downstream regions. This grouping provides valuable information for gene insertion in this chromosomal region, as it facilitates universal application of the pRC system in RSSC strains. </jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (12)*注記

もっと見る

関連プロジェクト

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ