Enhancing Photostability of a Coumarin Dye by Self‐inclusion into a Cyclodextrin Cavity in Aqueous Solution and Living Cells

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  • Hajime Shigemitsu
    Department of Applied Chemistry Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita 565-0871 Japan
  • Keigo Matsuda
    Department of Applied Chemistry Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita 565-0871 Japan
  • Tadashi Mori
    Department of Applied Chemistry Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita 565-0871 Japan
  • Hirotaka Nakatsuji
    Department of Applied Chemistry Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita 565-0871 Japan
  • Michiya Matsusaki
    Department of Applied Chemistry Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita 565-0871 Japan
  • Toshiyuki Kida
    Department of Applied Chemistry Graduate School of Engineering Osaka University 2-1 Yamadaoka Suita 565-0871 Japan

説明

<jats:title>Abstract</jats:title><jats:p>Photostable organic dyes are highly demanded for longtime or super‐resolution bioimaging. Herein, we demonstrate effective improvement of photostability of a coumarin dye (Pacific Blue (<jats:bold>PB</jats:bold>)) by steric protection using a cyclodextrin. The <jats:bold>PB</jats:bold> conjugated cyclodextrin (<jats:bold>PB−CD</jats:bold>) showed 2.8 times higher photostability than PB ethyl amide (<jats:bold>PB−EA</jats:bold>) which is a comparative compound in vitro. The cyclodextrin conjugation to <jats:bold>PB</jats:bold> not only protect <jats:bold>PB</jats:bold> unit from reactive chemicals, but also suppress reactive oxygen species (ROS) generation which causes dye degradation. The effects afford significant improvement of photostability of <jats:bold>PB</jats:bold> dye. Finally, the photostability of <jats:bold>PB−CD</jats:bold> was evaluated using confocal laser scanning microscopy (CLSM) and the significant improvement effect was proofed even in living cells.</jats:p>

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