The effect of repeated bouts of electrical stimulation‐induced muscle contractions on proteolytic signaling in rat skeletal muscle
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- Takaya Kotani
- Department of Life Sciences Graduate School of Arts and Sciences The University of Tokyo Tokyo Japan
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- Junya Takegaki
- Ritsumeikan Global Innovation Research Organization Ritsumeikan University Shiga Japan
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- Yuki Tamura
- Graduate School of Health and Sport Science Nippon Sport Science University Tokyo Japan
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- Karina Kouzaki
- Graduate School of Health and Sport Science Nippon Sport Science University Tokyo Japan
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- Koichi Nakazato
- Graduate School of Health and Sport Science Nippon Sport Science University Tokyo Japan
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- Naokata Ishii
- Department of Life Sciences Graduate School of Arts and Sciences The University of Tokyo Tokyo Japan
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説明
Mechanistic target of rapamycin complex 1 (mTORC1) plays a central role in muscle protein synthesis and repeated bouts of resistance exercise (RE) blunt mTORC1 activation. However, the changes in the proteolytic signaling when recurrent RE bouts attenuate mTORC1 activation are unclear. Using a RE model of electrically stimulated rat skeletal muscle, this study aimed to clarify the effect of repeated RE bouts on acute proteolytic signaling, particularly the calpain, autophagy-lysosome, and ubiquitin-proteasome pathway. p70S6K and rpS6 phosphorylation, indicators of mTORC1 activity, were attenuated by repeated RE bouts. Calpain 3 protein was decreased at 6 h post-RE in all exercised groups regardless of the bout number. Microtubule-associated protein 1 light chain 3 beta-II, an indicator of autophagosome formation, was increased at 3 h and repeated RE bouts increased at 6 h, post-RE. Ubiquitinated proteins were increased following RE, but these increases were independent of the number of RE bouts. These results suggest that the magnitude of autophagosome formation was increased following RE when mTORC1 activity was attenuated with repeated bouts of RE.
収録刊行物
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- Physiological Reports
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Physiological Reports 9 (9), 2021-05
Wiley