Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
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- Eiichi Murakami
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Jeryl Cheng
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Kira Gysel
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Zoltan Bozsoki
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Yasuyuki Kawaharada
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Christian Toftegaard Hjuler
- Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark
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- Kasper Kildegaard Sørensen
- Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark
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- Ke Tao
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Simon Kelly
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Francesco Venice
- Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
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- Andrea Genre
- Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
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- Mikkel Boas Thygesen
- Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark
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- Noor de Jong
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Maria Vinther
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Dorthe Bødker Jensen
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Knud Jørgen Jensen
- Department of Chemistry, University of Copenhagen, Frederiksberg, Denmark
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- Michael Blaise
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Lene Heegaard Madsen
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Kasper Røjkjær Andersen
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Jens Stougaard
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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- Simona Radutoiu
- Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
抄録
<jats:p>Recognition of Nod factors by LysM receptors is crucial for nitrogen-fixing symbiosis in most legumes. The large families of LysM receptors in legumes suggest concerted functions, yet only NFR1 and NFR5 and their closest homologs are known to be required. Here we show that an epidermal LysM receptor (NFRe), ensures robust signalling in L. japonicus. Mutants of Nfre react to Nod factors with increased calcium spiking interval, reduced transcriptional response and fewer nodules in the presence of rhizobia. NFRe has an active kinase capable of phosphorylating NFR5, which in turn, controls NFRe downstream signalling. Our findings provide evidence for a more complex Nod factor signalling mechanism than previously anticipated. The spatio-temporal interplay between Nfre and Nfr1, and their divergent signalling through distinct kinases suggests the presence of an NFRe-mediated idling state keeping the epidermal cells of the expanding root system attuned to rhizobia.</jats:p>
収録刊行物
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- eLife
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eLife 7 e33506-, 2018-06-29
eLife Sciences Publications, Ltd