β-Catenin is required for endothelial-mesenchymal transformation during heart cushion development in the mouse
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- Stefan Liebner
- 1FIRC Institute of Molecular Oncology, University of Milan, 20139 Milan, Italy
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- Anna Cattelino
- 1FIRC Institute of Molecular Oncology, University of Milan, 20139 Milan, Italy
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- Radiosa Gallini
- 1FIRC Institute of Molecular Oncology, University of Milan, 20139 Milan, Italy
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- Noemi Rudini
- 1FIRC Institute of Molecular Oncology, University of Milan, 20139 Milan, Italy
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- Monica Iurlaro
- 1FIRC Institute of Molecular Oncology, University of Milan, 20139 Milan, Italy
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- Stefano Piccolo
- 4Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, 35100 Padua, Italy
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- Elisabetta Dejana
- 1FIRC Institute of Molecular Oncology, University of Milan, 20139 Milan, Italy
書誌事項
- 公開日
- 2004-08-02
- DOI
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- 10.1083/jcb.200403050
- 公開者
- Rockefeller University Press
この論文をさがす
説明
<jats:p>During heart development endocardial cells within the atrio-ventricular (AV) region undergo TGFβ-dependent epithelial-mesenchymal transformation (EMT) and invade the underlying cardiac jelly. This process gives rise to the endocardial cushions from which AV valves and part of the septum originate. In this paper we show that in mouse embryos and in AV explants TGFβ induction of endocardial EMT is strongly inhibited in mice deficient for endothelial β-catenin, leading to a lack of heart cushion formation. Using a Wnt-signaling reporter mouse strain, we demonstrated in vivo and ex vivo that EMT in heart cushion is accompanied by activation of β-catenin/TCF/Lef transcriptional activity. In cultured endothelial cells, TGFβ2 induces α-smooth muscle actin (αSMA) expression. This process was strongly reduced in β-catenin null cells, although TGFβ2 induced smad phosphorylation was unchanged. These data demonstrate an involvement of β-catenin/TCF/Lef transcriptional activity in heart cushion formation, and suggest an interaction between TGFβ and Wnt-signaling pathways in the induction of endothelial-mesenchymal transformation.</jats:p>
収録刊行物
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- The Journal of Cell Biology
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The Journal of Cell Biology 166 (3), 359-367, 2004-08-02
Rockefeller University Press