Temporal Global Expression Data Reveal Known and Novel Salicylate-Impacted Processes and Regulators Mediating Powdery Mildew Growth and Reproduction on Arabidopsis
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- Divya Chandran
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Yu Chuan Tai
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Gregory Hather
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Julia Dewdney
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Carine Denoux
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Diane G. Burgess
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Frederick M. Ausubel
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Terence P. Speed
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
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- Mary C. Wildermuth
- Department of Plant and Microbial Biology (D.C., G.H., D.G.B., M.C.W.), Division of Biostatistics (Y.C.T.), and Department of Statistics (G.H., T.P.S.), University of California, Berkeley, California 94720; Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 (J.D., C.D., F.M.A.); and Department of Genetics, Harvard Medical School, Boston, Massachusetts 021
説明
<jats:title>Abstract</jats:title> <jats:p>Salicylic acid (SA) is a critical mediator of plant innate immunity. It plays an important role in limiting the growth and reproduction of the virulent powdery mildew (PM) Golovinomyces orontii on Arabidopsis (Arabidopsis thaliana). To investigate this later phase of the PM interaction and the role played by SA, we performed replicated global expression profiling for wild-type and SA biosynthetic mutant isochorismate synthase1 (ics1) Arabidopsis from 0 to 7 d after infection. We found that ICS1-impacted genes constitute 3.8% of profiled genes, with known molecular markers of Arabidopsis defense ranked very highly by the multivariate empirical Bayes statistic (T 2 statistic). Functional analyses of T 2-selected genes identified statistically significant PM-impacted processes, including photosynthesis, cell wall modification, and alkaloid metabolism, that are ICS1 independent. ICS1-impacted processes include redox, vacuolar transport/secretion, and signaling. Our data also support a role for ICS1 (SA) in iron and calcium homeostasis and identify components of SA cross talk with other phytohormones. Through our analysis, 39 novel PM-impacted transcriptional regulators were identified. Insertion mutants in one of these regulators, PUX2 (for plant ubiquitin regulatory X domain-containing protein 2), results in significantly reduced reproduction of the PM in a cell death-independent manner. Although little is known about PUX2, PUX1 acts as a negative regulator of Arabidopsis CDC48, an essential AAA-ATPase chaperone that mediates diverse cellular activities, including homotypic fusion of endoplasmic reticulum and Golgi membranes, endoplasmic reticulum-associated protein degradation, cell cycle progression, and apoptosis. Future work will elucidate the functional role of the novel regulator PUX2 in PM resistance.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 149 (3), 1435-1451, 2009-01-28
Oxford University Press (OUP)
